Illuminating Gene Expression with Single-Molecule Precision
Gene expression is determined not only by the quantity of mRNAs and proteins, but also by their precise localization within the cell. Using cutting-edge single-molecule imaging, our lab investigates the spatial regulation of gene expression, focusing on how cells accurately position mRNAs and proteins to ensure their proper function.
For instance, in yeast, approximately 20% of all the mRNAs are targeted to the surfaces of the endoplasmic reticulum (ER) and mitochondria, where they are translated to support organelle biogenesis and maintenance. In neurons, certain mRNAs are transported to synapses, where they play essential roles in synaptic activity and learning. Dysregulated mRNA localization in neurons is associated with neurological disorders, including fragile X syndrome (FXS) and amyotrophic lateral sclerosis (ALS).
We aim to uncover the mechanisms that regulate the spatial organization of gene expression, and determine the consequences of its dysregulation.